Journal: Oncotarget
Article Title: miR-331-3p and Aurora Kinase inhibitor II co-treatment suppresses prostate cancer tumorigenesis and progression
doi: 10.18632/oncotarget.18664
Figure Lengend Snippet: (A) Varying concentrations of AKi-II were used to establish an EC 50 of the inhibitor in LNCaP and 22Rv1 PCa cells. (B) LNCaP PCa cells were treated with 10 μM AKi-II over 5 days and proliferation was measured via cell titre. (C) The effect pretreatment of LNCaP PCa cells with miR-331-3p has on the EC 50 concentration of Aki-II. (D) Effects on colony formation of LNCaP PCa cells between miR-NC/miR-331-3p treated cells vs miR-NC/miR-331-3p and AKi-II treated cells. (E) Effects of si-RALA treatment on colony formation between si-RALA treated vs si-RALA and AKi-II treated 22Rv1 PCa cells. *p<0.05; CI=0.95; n=3, Error bars = SD.
Article Snippet: Trichostatin A (TSA) (Cat #T-8552) and 5-aza-2’-deoxycytidine (AZA) (Cat #A-3656) were from Sigma and Aurora Kinase inhibitor II (AKi-II) was from Santa Cruz Biotech (Cat #SC-203827).
Techniques: Concentration Assay